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2024

T. Kure, R. Ochiai, H. Sakai. Stability of Liposomal Membrane of Hemoglobin-Vesicles (Artificial Red Cells) for Over Years of Storage Evaluated Using Liquid Chromatography-Mass Spectrometry. Langmuir (in press).
DOI:10.1021/acs.langmuir.4c01403

I. Noguchi, H. Maeda, K. Kobayashi, T. Nagasaki, H. Kato, H. Yanagisawa, N. Wada, G. Kanazawa, T. Kaji, H. Sakai, S. Fujimaki, Y. Ono, K. Taguchi, V. T. G. Chuang, J. Saruwatari, M. Otagiri, H. Watanabe, T. Maruyama. Carbon monoxide-loaded cell therapy as an exercise mimetic for sarcopenia treatment. Free Radic. Biol. Med. 220, 67-77 (2024).
DOI:10.1016/j.freeradbiomed.2024.04.231

K. Nakano, M. Kohno, H. Onozawa, R. Hashimoto, K. Oiwa, R. Masuda, M. Yamaguchi, T. Hato, M. Watanabe, H. Horinouchi, H. Sakai, K. Kobayashi, M. Iwazaki. Using hemoglobin vesicles to treat operative hemorrhagic shock after pneumonectomy in dog models: an experimental study. Biomed. Res. 45(2), 91-101 (2024).
DOI:10.2220/biomedres.45.91

H. Sakai, T. Kure, N. Kobayashi, T. Ito, Y. Yamada, T. Yamada, R. Miyamoto, T. Imaizumi, J. Ando, T. Soga, Y. Osanai, M. Ogawa, T. Shimizu, T. Ishida, H. Azuma, Absence of anaphylactic reactions to injection of hemoglobin vesicles (artificial oxygen carriers) to rodents. ACS Omega. 9, 1904-1915 (2024).
DOI:10.1021/acsomega.3c08641




2023

Y. Syzuki, Y. Arakida, H. Sakai, Y. Enoki, K. Matsumoto, K. Taguchi, Elucidating the binding properties of methemoglobin in red blood cell to cyanide, hydrosulfide, and azide ions using artificial red blood cell. Toxycol. Appl. Pharmacol. 481(15), 116752 (2023).
DOI:10.1016/j.taap.2023.116752

T. Nagasai, H. Maeda, H. Yanagisawa, K. Nishida,K. Kobayashi, N. Wada, I. Noguchi, R. Iwakiri, K. Taguchi, H. Sakai, J. Saruwatari, H. Watanabe, M. Otagiri, T. Maruyama, Carbon monoxide-loaded red blood cell prevents the onset of cisplatin-induced acute kidney injury. Antioxidants 12(9), 1705 (2023).
DOI:10.3390/antiox12091705

N. K. M. Zin, H. Bochimoto, D. Kondoh, Y. Ishihara, H. Iwata, T. Shonaka, H. Obara, H. Sakai, H. Furukawa, N. Matsuno, Machine perfusion preservation with hemoglobin based oxygen vesicles alleviate ultrastructural damages in porcine liver donated after cardiac death. Microsc. Res. Tech. 86, 1725-1732 (2023).
DOI:10.1002/jemt.24405

T. Ochi, H. Nishiofuku, T. Kure, N. Saito, R. Taiji, N. Marugami, T. Tanaka, H. Sakai, Development of liposomal contrast agent with high iodine concentration and minimal effect on renal function. Biochem. Biophys. Rep. 34, 101473 (2023).
DOI:10.1016/j.bbrep.2023.101473

B. Takase, Y. Higashimura, H. Asahina, M. Ishihara, H. Sakai, Cardiac autonomic activity in a hemorrhagic shock-induced rat heart model. Curr. Med. Sci. 43, 232-245 (2023).
DOI:10.1007/s11596-023-2706-9


N. Rikihisa, K. Shimanouchi, Y. Saito, H. Sakai, N. Mitsukawa, Carbon monoxide combined with artificial blood cells acts as an antioxidant for tissues that have been thermal-damaged by dye laser irradiation. Burns 49(2), 388-400 (2023).
DOI:10.1016/j.burns.2022.03.009


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DOI:10.11638/jssmn.57.4_100

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2022

H. Sakai, T. Kure, K. Taguchi, H. Azuma, Research of storable and ready-to-use artificial red blood cells (hemoglobin vesicles) for emergency medicine and other clinical applications. Front. Med. Technol. 4, 1048951 (2022).
DOI:10.3389/fmedt.2022.1048951

H. Sakai, S. Yasuda, C. Okuda, T. Yamada, K. Owaki, Y. Miwa, Examination of central nervous system by functional observation battery after massive intravenous infusion of carbon monoxide-bound and oxygen-bound hemoglobin vesicles in rats. CRPHAR 3, 100135 (2022).
DOI:10.1016/j.crphar.2022.100135

K. Yamamoto
, H. Tanaka, G. Kurisu, R. Nakano, H. Yano, H. Sakai, Structural insights into the substrate specificity of IMP-6 and IMP-1 metallo-ƒÀ-lactamases. J. Biochem. 173(1), 21-30 (2022).
DOI:10.1093/jb/mvac080

H. Onozawa, M. Kohno, R. Hashimoto, K. Oiwa, R. Masuda, M. Yamaguchi, T. Hato, M. Watanabe, H. Horinouchi, H. Sakai, K. Kobayashi, M. Iwazaki, Early reperfusion with hemoglobin vesicles into tracheal subepithelial capillaries in a mouse tracheal transplant model. Biomed. Res. 43(4), 137-144 (2022).
DOI:10.2220/biomedres.43.137

H. Azuma, T. Amano, N. Kamiyama, N. Takehara, M. Jingu, H. Takagi, O. Sugita, N. Kobayashi, T. Kure, T. Shimizu, T. Ishida, M. Matsumoto, H. Sakai, First-in-human phase 1 trial of artificial red blood cells and hemoglobin vesicles developed as a transfusion alternative. Blood Adv. 6(21), 5711-5715 (2022).
DOI:10.1182/bloodadvances.2022007977

S. Ghirmai, H. Wu, M. Axelsson, T. Matsuhira, H. Sakai, I. Undeland, Exploring how plasma- and muscle-related parameters affect trout hemolysis as a route to prevent hemoglobin-mediated lipid oxidation of fish muscle. Sci. Rep. 12, 13446 (2022).
DOI:10.1038/s41598-022-16363-4

Y. Suzuki, K. Taguchi, T. Kure, Y. Enoki, M. Otagiri, H. Sakai, K. Matsumoto, Liposomal methemoglobin as a potent antidote for hydrogen sulfide poisoning. Toxicol. Appl. Pharmacol. 450(1), 116159 (2022).
DOI:10.1016/j.taap.2022.116159

T. Nagasaki, H. Maeda, K. Taguchi, H. Yanagisawa, K. Nishida, K. Kobayashi, N. Wada, I. Noguchi, R. Murata, H. Sakai, H. Kitagishi, J. Saruwatari, H. Watanabe, M. Otagiri, T. Maruyama, A bioinspired carbon monoxide delivery system prevents acute kidney injury and the progression to chronic kidney disease. Redox Biology, 54, 102371 (2022).
DOI:10.1016/j.redox.2022.102371

Y. Yuki, K. Hasegawa, M. Kinoshita, H. Ishibashi, K. Kaneko, O. Ishida, D. Saitoh, H. Sakai, K. Terui, Efficacy of resuscitative infusion with hemoglobin vesicles in rabbits with massive obstetric hemorrhage. Obstet. Anesth. Dig. 42(1) 18-19 (2022).
DOI:10.1097/01.aoa.0000816788.33546.05

Y. Suzuki, K. Taguchi, S. Hanyu, T. Kure, Y. Enoki, M. Otagiri, H. Sakai, K. Matsumoto, Oxidized liposomal artificial red blood cells rescue azide-poisoned mice from lethal toxidrome by recovering cytochrome c oxidase activity. J. Drug Deliv.Sci. Technol. 71, 103282 (2022).
DOI:10.1016/j.jddst.2022.103282

C. Okuda, H. Sakai, Effect of carbon monoxide administration using haemoglobin-vesicles on the hippocampal tissue. Artif. Cells Nanomed, Biotech. 50(1), 1-9 (2022).
DOI:10.1080/21691401.2022.2027428

B. Takase, Y. Higashimura, H. Asahina, N. Masaki, M. Kinoshita, H. Sakai, Intraosseous infusion of liposome-encapsulated hemoglobin (HbV) acutely prevents hemorrhagic anemia-induced lethal arrhythmias and its efficacy persists with preventing proarrhythmic side effects in subacute phase of severe hemodilution model. Artif. Organs 46(6), 1107-1121 (2022).
DOI:10.1111/aor.14170

K. Taguchi, Y. Suzuki, M. Tsutsuura, K. Hiraoka, Y. Watabe, Y. Enoki, M. Otagiri, H. Sakai, K. Matsumoto, Liposomal Artificial Red Blood Cell-Based Carbon Monoxide Donor Is a Potent Renoprotectant against Cisplatin-Induced Acute Kidney Injury. Pharmaceutics, 14(1), 57 (2022).
DOI:10.3390/pharmaceutics14010057

H. Usuda, M. Saito, H. Ikeda, S. Sato, Y. Kumagai, Y. Saito, S. Kawamura, T. Hanita, H. Sakai, S. Kure, N. Yaegashi, J. P. Newnham, M. W. Kemp, S. Watanabe, Assessment of synthetic red cell therapy for extremely preterm ovine fetuses maintained on an artificial placenta life-support platform. Artif. Organs 46(4), 653-665 (2022).
DOI:10.1111/aor.14155

Y. Watabe, K. Taguchi, H. Sakai, Y. Enoki, T. Maruyama, M. Otagiri, M Kohno, K. Matsumoto, Bioinspired carbon monoxide delivery using artificial blood attenuates the progression of obliterative bronchiolitis via suppression of macrophage activation by IL-17A. Eur. J. Pharm. Biopharm. 170, 43-51 (2022).
DOI:10.1016/j.ejpb.2021.11.011

T. Matsuhira
, H. Sakai, Artificial oxygen carriers, from nanometer- to micrometer-sized particles, made of hemoglobin composites substituting for red blood cells. Particuology 64, 43-55 (2022).
DOI:10.1016/j.partic.2021.08.010

H. Sakai, N. Kobayashi, T. Kure, H. Azuma. Potential Clinical Application of Hemoglobin Vesicles as an Artificial Oxygen Carrier and Carbon Monoxide Carrier. In: gBlood Substitutes and Oxygen Biotherapeuticsh (Eds. by H. Liu, A.D. Kaye, J.S. Jahr), Springer Nature Switzerland AG. Switzerland, pp. 235-242 (2022).
DOI:10.1007/978-3-030-95975-3_22

ŽðˆäG…, ¬—Ñ’¼Žq, ‹vâX’qŽq. ”õ’~E‹Ù‹}“Š—^‚ª‰Â”\‚ȐlHÔŒŒ‹…»Ü‚ÌŠJ”­ó‹µ. “ú–{ŽY•wl‰ÈEV¶Ž™ŒŒ‰tŠw‰ïŽ 31(2), 127-133 (2022).

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ISBN-13:978-4-9912052-0-0

ìˆä³—YCŽR–{œ¨ŽODˆä–{–«”ŽŽm ‘²Žõ‚Ì–â‚¢D‰»Šw‚ƍH‹Æ 75(3), 220-221 (2022)DƒŠƒ“ƒN

ŽðˆäG…D”õ’~E‹Ù‹}“Š—^‚ª‰Â”\‚ȐlHÔŒŒ‹…»Ü‚ÌŽÀ—p‰»‚ð–ÚŽw‚·Œ¤‹†. –Œ(MEMBRANE) 47(5), 246-251 (2022)D
DOI:10.5360/membrane.47.246


2021

H. Ishibashi, K. Hagisawa, M. Kinoshita, Y. Yuki, M. Miyamoto, T. Kure, H. Sakai, D. Saitoh, K. Terui, M. Takano, Resuscitative efficacy of hemoglobin vesicles for severe postpartum hemorrhage in pregnant rabbits. Sci. Rep. 11, 22367 (2021).
DOI:10.1038/s41598-021-01835-w

Y. Suzuki, K. Taguchi, T. Kure, Y. Enoki, M. Otagiri, H. Sakai, K. Matsumoto, Long-term pharmaceutical stability of liposome-encapsulated methemoglobin as an antidote for cyanide poisoning. Int. J. Pharm. 610(15), 121260 (2021).
DOI:10.1016/j.ijpharm.2021.121260

H. Iwata, A. Toriumi, D. Ishii, A. Yamamoto, Y. Sato, H. Takanashi, H. Yokoo, H. Obara, Y. Nishikawa, H. Sakai, N. Matsuno, Applicability of hemoglobin vesicles as transplantable liver perfusion preservation solution. Artif. Blood 29(1), 43-49 (2021).

Y. Suzuki, K. Taguchi, T. Kure, H. Sakai, Y. Enoki, M. Otagiri, K. Matsumoto, Liposome-encapsulated methemoglobin as an antidote against cyanide poisoning. J. Control. Release 337, 59-70 (2021).
DOI:10.1016/j.jconrel.2021.07.015

B.Takase, Y. Higashimura, H. Asahina, M. Ishihara, H. Sakai, Liposome-encapsulated hemoglobin (HbV) transfusion rescues rats undergoing progressive lethal 85% hemorrhage as a result of an anti-arrhythmogenic effect on the myocardium. Artificial@Organs 45, 1391-1404 (2021).
DOI:10.1111/aor.14033

T. Kure, H. Sakai, Preparation of Artificial Red Blood Cells (Hemoglobin Vesicles) Using the Rotation-Revolution Mixer for High Encapsulation Efficiency. ACS Biomater. Sci. Eng. 7(6), 2835-2844 (2021).
DOI:10.1021/acsbiomaterials.1c00424


H. Sakai
, N. Kobayashi, T.Kure, C. Okuda, Translational research of hemoglobin vesicles as a transfusion alternative. Curr. Med. Chem. 29(3), 591-606 (2021).
DOI:10.2174/0929867328666210412130035

T. Matsuhira, H. Sakai, Entropy-driven supramolecular ring-opening polymerization
of a cyclic hemoglobin monomer for constructing a hemoglobin-PEG alternating
polymer with structural regularity. Biomacromolecules 22(5), 1944-1954 (2021).
DOI:10.1021/acs.biomac.1c00061

K. Hagisawa, M. Kinoshita, H. Sakai, S. Takeoka, Artificial blood transfusion. Physiology News 121, 22-25. DOI:10.36866/pn.121.22

https://static.physoc.org/app/uploads/2021/03/29093550/PN121_ONLINE_HR.pdf

K. Shimanouchi, N. Rikihisa, Y. Saito, N. Tsumura, H. Sakai, N. Mitsukawa, Artificial red blood cells increase large vessel wall damage and decrease surrounding dermal tissue damage in a rabbit auricle model after subsequent flashlamp]pumped pulsed]dye laser treatment. J. Dermatol. 48(5), 600-612 (2021).
DOI:10.1111/1346-8138.15805

Y. Yuki, K. Hagisawa, M. Kinoshita, H. Ishibashi, K. Kaneko, O. Ishida, D. Saitoh, H. Sakai, K. Terui, Efficacy of Resuscitative Infusion with Hemoglobin Vesicles in Rabbits with Massive Obstetric Hemorrhage. Am. J. Obstet. Gynecol. 224(4), 398.e1-398.e11 (2021). DOI:10.1016/j.ajog.2020.09.010

T.M.S. Chang, L. Bülow, J.S. Jahr, H. Sakai, C. Yang (eds.), Nanobiotherapeutic Based Blood Substitutes (Regenerative Medicine, Artificial Cells and Nanomedicine), World Scientific Publishing Co. Pte. Ltd., Singapore (2021).
ISBN-13:978-981-122-868-1
DOI:10.1142/12054
(Chapter 3.9) Analysis of dimeric ƒ¿ƒÀ subunit exchange between bis-PEGylated and native hemoglobins. pp 417-431.
T. Matsuhira, K. Yamamoto, H. Sakai

(Chapter 3.10) Ring-opening polymerization of hemoglobin based on supramolecular chemistry. pp 433-445.
T. Matsuhira, K. Yamamoto, H. Sakai

(Chapter 6.7) Translational research of hemoglobin vesicles (artificial red cells) for a transfusion alternative and O2/CO therapeutics. pp 811-822.
H. Sakai, H. Azuma, H. Horinouchi, K. Kobayashi

(Chapter 6.8) Hemoglobin vesicles as a transfusion alternative for perioperative hemorrhage in thoracic surgery. pp 823-835.
M. Kohno, R. Hashimoto, H. Onozawa, K. Oiwa, H. Horinouchi, H. Sakai, K. Kobayashi, M. Iwazaki

(Chapter 6.9) Prevention of methemoglobin formation in artificial red cells (hemoglobin vesicles). pp 837-847.
H. Sakai, M. Yamada

(Chapter 6.10) Carbon monoxide-bound hemoglobin vesicles: Current facts and potential medical applications. pp 849-866.
K. Taguchi, K. Matsumoto, H. Sakai, T. Maruyama, M. Otagiri

(Chapter 6.11) Influence of hemoglobin vesicles on immune function. pp 867-877.
H. Azuma, M. Fujihara, H. Sakai

(Chapter 6.12) Membrane fluidity of artificial red cells encapsulating a concentrated hemoglobin solution possessing a high colloid osmotic pressure. pp 879-888.
T. Kure, H. Sakai

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2020

Y. Yoshida, T. Nagamori, E. Ishibazawa, H. Kobayashi, T. Kure, H. Sakai, D. Takahashi, M. Fujihara, H. Azuma, Contribution of long chain fatty acid to induction of myeloid-derived suppressor cell (MDSC)-like cells - Induction of MDSC by lipid vesicles (liposome) - Immunopharmacol. Immunotoxicol. 42(6), 614-624 (2020). DOI:10.1080/08923973.2020.1837866

M. Tokuno, K. Taguchi, H. Sakai, S. Ohtsuki, K. Yamasaki, M. Otagiri, Assessing cytochrome P450-based drug-drug interactions with Hemoglobin-vesicles, an artificial red blood cell preparation, in healthy rats. Drug Metab. Pharmacokinet. 35(5), 425-431 (2020). DOI:10.1016/j.dmpk.2020.06.005

M. Tokuno, K. Taguchi, H. Sakai, S. Ohtsuki, K. Yamasaki, M. Otagiri, Evaluation of cytochrome P450-based drug metabolism in hemorrhagic shock rats that were transfused with native and an artificial red blood cell preparation, Hemoglobin-vesicles. Drug Metab. Pharmacokinet. 35(5), 417-424 (2020). DOI:10.1016/j.dmpk.2020.06.004

K. Hagisawa, M. Kinoshita, D. Saitoh, Y. Morimoto, H. Sakai, Intraosseous transfusion of hemoglobin vesicles in the treatment of hemorrhagic shock with collapsed vessels in a rabbit model. Transfusion 60(7), 1400-1409 (2020). DOI:10.1111/trf.15915

R. Hashimoto, M. Kohno, K. Oiwa, H. Onozawa, M. Watanabe, H. Horinouchi, H. Sakai, K. Kobayashi, M. Iwazaki, Immediate effects of systemic administration of normal and high O2-affinity haemoglobin vesicles as a transfusion alternative in a rat pneumonectomy model. BMJ Open Resp. Res. 7(1), e000476 (2020). DOI:10.1136/bmjresp-2019-000476

ŽðˆäG…. lHÔŒŒ‹…(ƒwƒ‚ƒOƒƒrƒ“ƒxƒVƒNƒ‹)»Ü‚É‚æ‚éoŒŒ«ƒVƒ‡ƒbƒNŽ¡—Ẩ”\«. ŠO‰È‚Æ‘ãŽÓE‰h—{ 54(4), 170-174 (2020).
DOI:10.11638/jssmn.54.4_170


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2019

T. Shonaka, N. Matsuno, H. Obara, R. Yoshikawa, Y. Nishikawa, Y. Ishihara, H. Bochimoto, M. Gochi, M. Otani, H. Kanazawa, H. Azuma, H. Sakai, Hiroyuki Furukawa, Impact of human-derived hemoglobin based oxygen vesicles as a machine perfusion solution for liver donation after cardiac death in a pig model. PLOS ONE 14(12), e0226183 (2019). DOI:10.1371/journal.pone.0226183

H. Sakai, C. Leong, Prolonged functional life span of artificial red cells in blood circulation by repeated methylene blue injections. Artif. Cells Nanomed. Biotechnol. 47(1), 3123-3128 (2019). DOI:10.1080/21691401.2019.1645157

K. Hagisawa, M. Kinoshita, M. Takikawa, S. Takeoka, D. Saitoh, S. Seki, H. Sakai, Combination therapy using fibrinogen ƒÁ]chain peptide]coated, ADP]encapsulated liposomes and hemoglobin vesicles for trauma]induced massive hemorrhage in thrombocytopenic rabbits. Transfusion 59(10), 3186-3196 (2019). DOI:10.1111/trf.15427

M. Yamada, T. Matsuhira, K. Yamamoto, H. Sakai, Antioxidative pseudo-enzymatic mechanism of NAD(P)H coexisting with oxyhemoglobin for suppressed methemoglobin formation. Biochemistry 58(10), 1400-1410 (2019). DOI:10.1021/acs.biochem.8b01314

T. Matsuhira, K. Yamamoto, H. Sakai, Ring-opening polymerization of hemoglobin. Biomacromolecules 20(4), 1592-1602 (2019). DOI:10.1021/acs.biomac.8b01789

B. Takase, Y. Higashimura, K. Hashimoto, H. Asahina, M. Ishihara, H. Sakai, Myocardial Electrical Remodeling and the Arrhythmogenic Substrate in Hemorrhagic Shock-Induced Heart: Anti-Arrhythmogenic Effect of Liposome-Encapsulated Hemoglobin (HbV) on the Myocardium. Shock 52(3), 378-386 (2019). DOI: 10.1097/SHK.0000000000001262

‚£–}•½, “Œ‘º—IŽq, ’©”ä“Þ‚Í‚é‚©, ŽðˆäG…. oŒŒ«ƒVƒ‡ƒbƒNS‘Ÿ‚̍Õs®–¬«ì—p‚É‹y‚Ú‚·lHÔŒŒ‹…‚Ì–«Œø‰Ê. Therapeutics Research 40(9), 709-716 (2019). ƒŠƒ“ƒN

‚£–}•½, ‹´–{Œ«ˆê, “Œ‘º—IŽq, ’©”ä“Þ‚Í‚é‚©, ŽðˆäG…. ŒõHŠw“IŽè–@‚É‚æ‚郊ƒ|ƒ\[ƒ€••“üŽ_‘f‰^”À‘Ì‚Ì’vŽ€«oŒŒ«ƒVƒ‡ƒbƒN(85%oŒŒŒã‘h¶)ŽÀŒ±ƒ‚ƒfƒ‹‚É‚¨‚¯‚éR•s®–¬Œø‰Ê‚ÉŠÖ‚·‚錟“¢. Therapeutics Research 40(4), 227-284 (2019). ƒŠƒ“ƒN

“ŒŠ°CŽðˆäG…. lHÔŒŒ‹…iƒwƒ‚ƒOƒƒrƒ“ƒxƒVƒNƒ‹j‚̗Տ°‚Ö‚Ì‹´“n‚µŒ¤‹†. —Տ°ŒŒ‰t 60(9), 1084-1091 (2019). DOI:10.11406/rinketsu.60.1084


2018

T. Matsuhira, T. Kure, K. Yamamoto, H. Sakai, Analysis of dimeric ƒ¿ƒÀ subunit exchange between PEGylated and native hemoglobins (ƒ¿2ƒÀ2 tetramer) in an equilibrated state by intramolecular ƒÀƒÀ-crosslinking. Biomacromolecules 19(8), 3412-3420 (2018). DOI: 10.1021/acs.biomac.8b00728

H. Sengyoku, T. Tsuchiya, T. Obata, R. Doi, Y. Hashimoto, M. Ishii, H. Sakai, N. Matsuo, D. Taniguchi, T. Suematsu, M. Lawn, K. Matsumoto, T. Miyazaki, T Nagayasu, Sodium hydroxide based non-detergent decellularizing solution for rat lung. Organogenesis 14(2), 94-106 (2018). DOI: 10.1080/15476278.2018.1462432

K. Taguchi, S. Nagao, H. Maeda, H. Yanagisawa, H. Sakai, K. Yamasaki, T. Wakayama, H. Watanabe, M. Otagiri, T. Maruyama, Biomimetic carbon monoxide delivery based on hemoglobin vesicles ameliorates acute pancreatitis in mice via the regulation of macrophage and neutrophil activity. Drug Delivery 25(1), 1266-1274 (2018). DOI: 10.1080/10717544.2018.1477860

N. Rikihisa, M. Tomonaga, S. Watanabe, Y. Saito, H. Sakai, Intravenous injection of artificial red cells and subsequent dye laser irradiation causes deep vessel impairment in an animal model of port-wine stain. Lasers Med. Sci. 33(6), 1287-1293 (2018). DOI: 10.1007/s10103-018-2480-2

T. Shonaka, N. Matsuno, H. Obara, R. Yoshikawa, Y. Nishikawa, M. Gouchi, M. Otani, H. Takahashi, H. Azuma, H. Sakai, H. Furukawa, The application of perfusate with human-derived oxygen carrier solution under subnormothermic machine perfusion for donation after cardiac death (DCD) liver grafts in pigs. Transp. Proc. 50(9), 2821-2825 (2018). DOI: 10.1016/j.transproceed.2018.02.184

K. Hagisawa, M. Kinoshita, B. Takase, K. Hashimoto, D. Saitoh, S. Seki, Y. Nishida, H. Sakai, Efficacy of Resuscitative Transfusion with Hemoglobin Vesicles in the Treatment of Massive Hemorrhage in Rabbits with Thrombocytopenic Coagulopathy and its Effect on Hemostasis by Platelet Transfusion. Shock 50(3), 324-330 (2018). DOI: 10.1097/SHK.0000000000001042

¯’†’B–çC¼–ì’¼“kC¬Œ´O“¹C‹gì—ɁC¼ì—SŽiC‡’n”üŽqC‘å’J«GC‚‹´—T”VC“ŒŠ°CŽðˆäG…CŒÃì”Ž”V. S’âŽ~ƒhƒi[‚ւ̊̈ڐA‚ɑ΂·‚élHÔŒŒ‹…(Hemoglobin vesicles, HbV)‚ð—p‚¢‚½Ÿó—¬•Û‘¶•û–@‚Ì—LŒø«. –kŠC“¹ŠO‰ÈŽGŽ 63(2), 46-48 (2018).

ŽðˆäG…C‰œ“cçˆ¤C‹vâX’qŽq. lHÔŒŒ‹…‚ÌŠJ”­‚ƗՏ°‰ž—p. LiSA 25(11), 1139-1146 (2018).

ŽðˆäG…. —AŒŒ‘ã‘Ö‚Ì‘¼‚É‚à‚³‚Ü‚´‚Ü‚È—˜—p‚ªŠú‘Ò‚³‚ê‚éulHÔŒŒ‹…v‚Æ‚Í. Medical Technology 46 (9), 832-834 (2018).

H. Sakai, Artificial red cells (hemoglobin vesicles) as a transfusion alternative and their new clinical applications. Japanese Journal of Transfusion and Cell Therapyt 64(4), 589-596 (2018). (in Japanese) ŽðˆäG…. lHÔŒŒ‹…iƒwƒ‚ƒOƒƒrƒ“ ƒxƒVƒNƒ‹j»Ü‚̐V‚µ‚¢—˜—p–@. “ú–{—AŒŒ×–EŽ¡—Êw‰ïŽGŽ 64(4), 589-596 (2018).

T. Kure, H. Sakai, The measurement of the lipid membrane fluidity of liposomes encapsulating a concentrated protein solution using a fluorescence polarization technique. Jasco report 60(2), 5-12 (2018). (in Japanese) ‹vâX’qŽqCŽðˆäG…. ŒuŒõ•ÎŒõ‰ðÁ–@‚É‚æ‚鍂”Z“x’`”’Ž¿—n‰t‚ð“à•ï‚µ‚½ƒŠƒ|ƒ\[ƒ€Ž‰Ž¿–Œ‚Ì—¬“®«•]‰¿. Jasco Report 60(2), 5-12 (2018).

ŽðˆäG…. ”pŠü‚·‚錌‰t‚©‚çlHÔŒŒ‹…‚ð‚‚­‚éB’·Šú•Û‘¶‰Â”\AŒ£ŒŒ•s‘«‚̉ۑè‰ðŒˆ‚É. ƒRƒ“ƒo[ƒeƒbƒN 541, 20-23 (2018).

ŽðˆäG…C‘¾“c‰pLC¬—эhˆêC”ªdŠ~L¶. lHÔŒŒ‹…iƒwƒ‚ƒOƒƒrƒ“ƒxƒVƒNƒ‹j. “ú–{ŽY•wl‰Èˆã‰ïAŒ¤Cƒm[ƒgNo. 100uŽY•wl‰Èˆã—Â̋ߖ¢—ˆv 67-69 (2018”N 1ŒŽ).


2017

S. Ghirmai, L. Bulow, H. Sakai, In vivo evaluation of electron mediators for the reduction of methemoglobin encapsulated in liposomes using electron energies produced by red blood cell glycolysis. Artif. Cells Nanomed. Biotechnol. 46, 1364-1372 (2017). DOI: 10.1080/21691401.2017.1397003

H. Ohta, M. Kaga, H. Li, H. Sakai, K. Okamura, N. Yaegashi, Potential New Non-Invasive Therapy Using Artificial Oxygen Carriers for Pre-Eclampsia. J. Funct. Biomater. 8, 32 (2017). DOI: 10.3390/jfb8030032

H. Azuma, M. Fujihara, H. Sakai, Biocompatibility of HbV: Liposome-Encapsulated Hemoglobin Molecules-Liposome Effects on Immune Function. J. Funct. Biomater. 8, 24 (2017). DOI: 10.3390/jfb8030024

M. Kohno, T. Ikeda, R. Hashimoto, Y. Izumi, M. Watanabe, H. Horinouchi, H. Sakai, K. Kobayashi, M. Iwazaki, Acute 40% exchange-transfusion with hemoglobin-vesicles in a mouse pneumonectomy model. PLOS ONE 12(6), e0179724 (2017). DOI: 10.1371/journal.pone.0178724

M. Yamada, H. Sakai. Hidden Antioxidative Functions of NADH Coexisting with Hemoglobin. ACS Chem. Biol. 12(7), 1820-1829 (2017). DOI: 10.1021/acschembio.7b00174

K. Taguchi, K. Yamasaki, H. Sakai, T. Maruyama, M. Otagiri, The use of Hemoglobin-vesicles for delivering medicinal gas for the treatment of intractable disorders. J. Pharmaceut. Sci. 106(9), 2392-2400 (2017). DOI: 10.1016/j.xphs.2017.04.006

H. Azuma, Y, Yoshida, H. Takahashi, E. Ishibazawa, H. Kobayashi, H. Sakai, D. Takahashi, M. Fujihara. Liposomal microparticle injection can induce myeloid-derived suppressor cells (MDSC)-like cells in vivo. Immunopharmacol. Immunotoxicol. 39, 140-147 (2017). DOI: 10.1080/08923973.2017.1306867

N. Rikihisa, S. Watanabe, Y. Saito, H. Sakai. Artificial Red Blood Cells as Potential Photosensitizers in Dye Laser Treatment Against Port-Wine Stains. J. Funct. Biomater. 8, 14, (2017). DOI: 10.3390/jfb8020014

H. Sakai. Overview of potential clinical applications of hemoglobin vesicles (HbV) as artificial red cells, evidenced by preclinical studies of the academic research consortium. J. Funct. Biomater. 8, 10 (2017). DOI: 10.3390/jfb8010010

Y. Naito, H. Sakai, S. Inoue, M. Kawaguchi. Hemoglobin vesicles prolong the time to circulatory collapse in rats during apnea. BMC Anesthesiol. 17, 44 (2017). DOI: 10.1186/s12871-017-0338-y

N. Rikihisa, S. Watanabe, K. Satoh, Y. Saito, H. Sakai. Photosensitizer Effects of Artificial Red Cells on Dye Laser Irradiation in an Animal Model Assuming Port-Wine Stain Treatment. Plast. Reconstr. Surg. 139, 707e-716e (2017). DOI: 10.1097/PRS.0000000000003082

T. Kure, H. Sakai. Transmembrane difference in colloid osmotic pressure affects the lipid membrane fluidity of liposomes encapsulating a concentrated hemoglobin solution. Langmuir 33, 1533-1540 (2017). DOI: 10.1021/acs.langmuir.6b04643


2016

M. Tokuno, K. Taguchi, K. Yamasaki, H. Sakai, M. Otagiri. Long-term stored hemoglobin-vesicles, a cellular type of hemoglobin-based oxygen carrier, has resuscitative effects comparable to that for fresh red blood cells in a rat model with massive hemorrhage without post-transfusion lung injury. PLoS ONE 11(10), e0165557 (2016).

S. Nagao, K. Taguchi, H. Sakai, K. Yamasaki, H. Watanabe, M. Otagiri, T. Maruyama. Carbon monoxide-bound hemoglobin vesicles ameliorate multiorgan injuries induced by severe acute pancreatitis in mice by their anti-inflammatory and antioxidant properties. Int. J. Nanomedicine 11, 5611-5620 (2016).

S. Nagao, K. Taguchi, Y. Miyazaki, T. Wakayama T, VT. Chuang, K. Yamasaki, H. Watanabe, H. Sakai, M. Otagiri, T. Maruyama. Evaluation of a new type of nano-sized carbon monoxide donor on treating mice with experimentally induced colitis. J. Control. Release 234, 49-58 (2016).

S. Osaki, K. Yamamoto, T. Matsuhira, H. Sakai. The effects of seasonal changes on the molecular weight of Nephila clavata spider silk. Polymer Journal 48, 659-653 (2016). DOI: 10.1038/pj.2015.138

ŽðˆäG…(–|–󕪒S). ƒn[ƒo[E¶‰»ŠwiŒ´‘30”Łj2016/11/30 ŠÛ‘P 53Í(799-811) ´…F—Y(ŠÄ–ó).

‘¾“c‰pLC‰Á‰ê–ƒˆßŽqC—›ƒRƒECŽðˆäG…C‰ª‘ºB”ŽC”ªdŠ~L¶. lHŽ_‘f‰^”À‘Ì‚Í”DP‚ŒŒˆ³ÇŒóŒQƒ‰ƒbƒgƒ‚ƒfƒ‹‚Ì‘Ù”Õ’áŽ_‘fE‘ÙŽe”­ˆç•s‘S‚ðŽ¡—Ã‚·‚é. lHŒŒ‰t 24, 63-72 (2016).


2015

B. Namgung, H. Sakai, S. Kim. Influence of erythrocyte aggregation at pathological levels on cell-free marginal layer in a narrow circular tube. Clin. Hemorheol. Microcirculation 61, 445-457 (2015).

H. Li, H. Ohta, Y. Tahara, S. Nakamura, K. Taguchi, M. Nakagawa, Y. Oishi, Y. Goto, K. Wada, M. Kaga, M. Inagaki, M. Otagiri, H. Yokota, S. Shibata, H. Sakai, K. Okamura, N. Yaegashi. Artificial oxygen carriers rescue placental hypoxia and improve fetal development in the rat pre-eclampsia model. Sci. Rep. 5, 15271 (2015).

K. Taguchi, S. Nagao, K. Yamasaki, H. Sakai, H. Seo, T. Maruyama, M. Otagiri. Biological Responsiveness and Metabolic Performance of Liposome-Encapsulated Hemoglobin (Hemoglobin-Vesicles) in Apolipoprotein E-Deficient Mice after Massive Intravenous Injection. Biol. Pharm. Bull. 38, 1606-1616 (2015).

K. Kettisen, L. Bulow, H. Sakai. Potential electron mediators to extract electron energies of RBC glycolysis for prolonged in vivo functional lifetime of hemoglobin-vesicles. Bioconjugate Chem. 26, 746-754 (2015).

J. Araki, H. Sakai, D. Takeuchi, Y. Kagaya, M. Naito, M. Mihara, M. Narushima, T. Iida, I. Koshima. Normothermic preservation of the rat hind limb with artificial oxygen-carrying hemoglobin vesicles. Transplantation 99, 687-692 (2015).

T. Matsuhira, S. Osaki. Molecular weight of Nephila clavata spider silk. Polymer Journal, 47, 456-459 (2015). DOI: 10.1038/pj.2015.10

ŽðˆäG…, ‹vâX’qŽq. lHÔŒŒ‹…(ƒwƒ‚ƒOƒƒrƒ“ƒxƒVƒNƒ‹)‚ÌŽÀŒ»‚ÉŒü‚¯‚Ä. ˆãŠw‚Ì‚ ‚ä‚Ý 253, 1180-1181 (2015).


2014

H. Sakai, B. Li, W. Lim, Y. Iga. Red blood cells donate electrons to methylene blue mediated chemical reduction of methemoglobin compartmentalized in liposome in blood. Bioconjugate Chem. 25, 1301-1310 (2014).

S. Nagao, K. Taguchi, H. Sakai, R. Tanaka, H. Horinouchi, H. Watanabe, K. Kobayashi, M. Otagiri, T. Maruyama. Carbon monoxide-bound hemoglobin-vesicles as a potential therapeutic agent for the treatment of bleomycin-induced pulmonary fibrosis. Biomaterials 35, 6553-6562 (2014).

M. Fujihara, D. Takahashi, H. Abe, H. Sakai, H. Horinouchi, K. Kobayashi, H. Ikeda, H. Azuma. Primary and secondary immune responses to keyhole limpet hemocyanin in rats after the infusion of hemoglobin vesicle, an artificial oxygen carrier. Artif. Organs 38, 234-238 (2014) .

ŽðˆäG…, lHÔŒŒ‹…(ƒwƒ‚ƒOƒƒrƒ“¬–E‘Ì)”÷—±Žq•ªŽU‰t‚Ì“Á’¥. •²‘̍HŠw 6, 909-914 (2014).

ŽðˆäG…, lHÔŒŒ‹…‚É‚æ‚鐶‘Ì‘gD‚Ö‚ÌŽ_‘f—A‘—. u‘Sl—́E‰ÈŠw—́E“§Í—Í‚ÉŠî‚­“§ÍˆãŠwv‘æ6Í: t«•nŒŒ. pp.369-373A•½•ûGŽ÷ ŠÄCAˆã–òƒWƒƒ[ƒiƒ‹ŽÐ. ‘åã (2014).

ŽðˆäG…, lHÔŒŒ‹…‚ÌŠJ”­ó‹µ‚Ə«—ˆ“W–]. Anesthesia Network 18, 37-41 (2014).


2013

H. Sakai, K. Ng, B. Li, N. Sugimura. Swine hemoglobin as a potential source of artificial oxygen carriers, hemoglobin-vesicles. Artif. Cells Nanomed. Biotechnol. 41, 37-41 (2013).

H.W. Kim, J.S. Jahr, A. Mozzarelli, H. Sakai. International consortium for development of hemoglobin-based oxygen carriers, oxygen therapeutics and multifunctional resuscitation fluids-a white paper. In: Hemoglobin-based oxygen carriers-principles, approaches and current status (H.W. Kim & A.G. Greenburg eds.) Springer-Verlag (Berlin/Heidelberg, Germany). Chapter 39, pp. 737-746 (2013).

T. Ikeda, H. Horinouchi, H. Sakai. Cellular-type hemoglobin-based oxygen carriers as a resuscitative fluid for hemorrhagic shock: acute and long-term safety evaluation using beagle dogs. In: Hemoglobin-based oxygen carriers-principles, approaches and current status (H.W. Kim & A.G. Greenburg eds.) Springer-Verlag (Berlin/Heidelberg, Germany). Chapter 28, pp. 501-526 (2013).

H. Azuma, M. Fujihara, H. Sakai. Biocompatibility of hemoglobin vesicles, a cellular-type artificial oxygen carrier, on blood cells and plasma proteins in vitro and in vivo. In: Hemoglobin-based oxygen carriers-principles, approaches and current status (H.W. Kim & A.G. Greenburg eds.) Springer-Verlag (Berlin/Heidelberg, Germany). Chapter 22, pp. 385-398 (2013).

H. Sakai. Cellular-type hemoglobin-based oxygen carriers to mimic the red blood cells structure. In: Hemoglobin-based oxygen carriers-principles, approaches and current status (H.W. Kim & A.G. Greenburg eds.) Springer-Verlag (Berlin/Heidelberg, Germany). Chapter 12, pp. 235-248 (2013).

ŽðˆäG…C–x”V“àG‹vA“ŒŠ°A¬“cØ—DŽ÷A¬—эhˆê. —AŒŒ‘ã‘Ö‚Æ‚µ‚Ă̐lHÔŒŒ‹…(ƒwƒ‚ƒOƒƒrƒ“¬–E‘Ì)»Ü‚̈À‘S«ŽŽŒ±. lHŒŒ‰t 21, 36-48 (2013).

H. Sakai. Biocompatibility of a highly concentrated fluid of Hemoglobin-vesicles as a transfusion alternative. In: Selective Topics in Nanomedicine (T.M.S. Chang ed.), pp. 133-147, World Scientific, Singapore (2013).

T. Matsuhira, K. Yamamoto, S. Osaki. Effects of UV irradiation on the molecular weight of spider silk, Polymer Journal, 45, 1167-1169 (2013). DOI: 10.1038/pj.2013.41


2012

K. Taguchi, H. Watanabe, H. Sakai, H. Horinouchi, K. Kobayashi, T. Maruyama, M. Otagiri. Fourteen-days observation and pharmacokinetic evaluation after massive intravenous infusion of hemoglobin-vesicles (artificial oxygen carriers) in cynomolgus monkeys. J. Drug Metab. Toxicol. 3, 1000128 (2012).

M. Kaga, H. Ohta, Y. Lee, R. Kamii, H. Yamamoto, S. Akiyama, S. Watanabe, T. Matsuda, Y. Kimura, S. Tsuchiya, H. Tei, L. Okamura, H. Sakai, N. Yaegashi. Physiolgical capacity of the reticuloendothelial system for the degradation of hemoglobin-vesicles (artificial oxygen carriers) after massive intravenous doses by daily repeated infusion for 7 days in Pregnant rats and fetuses. Life Sci. 91, 420-428 (2012).

H. Sakai, Y. Suzuki, K. Sou, M. Kano. Cardiopulmonary hemodynamic responses to the small injection of hemoglobin-vesicles (artificial oxygen carriers) in miniature pigs. J. Biomed. Mater. Res. A, 100A, 2668-2677 (2012).

A.G. Tsai, M. Intaglietta, H. Sakai, E. Delpy, C.D. la Rochelle, M. Rousselot, F. Zal. Microcirculation and NO-CO studies of a natural extracellular hemoglobin developed for an oxygen therapeutic carrier. Current Drug Discovery Techonl. 9, 166-172 (2012).

Y. Seishi, H. Horinouchi, H. Sakai, K. Kobayashi. Effect of the cellular-type artificial oxygen carrier Hb-vesicle as a resuscitative fluid for pre-hospital treatment: Experiments in a rat uncontrolled hemorrhagic shock model. Shock 38, 153-158 (2012).

H. Sakai, K. Sou, H. Horinouchi, E. Tsuchida, K. Kobayashi. Removal of Hemoglobin-vesicles from circulating blood by using centrifugation and ultrafiltration. Artif. Organs 36, 202-209 (2012).

M. Yamamoto, H. Horinouchi, Y. Seishi, N. Sato, M. Itoh, K. Kobayashi, H. Sakai, Fluid resuscitation of hemorrhagic shock with Hemoglobin vesicles in Beagle dogs: pilot study. Artif. Cells Blood Substitutes Biotechnol. 40, 179-195 (2012).

T. Sato, T. Fukasawa, T. Komatsu, H. Sakai, S. Ishiwata. Protein-protein interactions in solution and their interplay with protein specific functions. J. Phys. Soc. Jpn 81 (suppl.), SA002-1 - SA-002-11 (2012).

H. Sakai. Cellular-type hemoglobin-based oxygen carrier (hemoglobin-vesicles) as a transfusion alternative and for oxygen therapeutics. Current Drug Discovery Techonl. 9, 188-193 (2012).


2011

H. Sakai, N. Miyagawa, H. Horinouchi, S. Takeoka, M. Takaori, E. Tsuchida, K. Kobayashi. Intravenous injection of Hb-vesicles (artificial oxygen carriers) after hemodilution with a series of plasma expanders (water-soluble biopolymers) in a rat repeated hemorrhage model. Polymers Adv. Technol. 22, 1216-1222 (2011).

A. Niwa, T. Heike, K. Umeda, K. Oshima, I. Kato, H. Sakai, H. Suemori, T. Nakahata, M.K. Saito. A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors". PLoS ONE 6, e22261 (2011).

K. Taguchi, S. Ogaki, H. Watanabe, D. Kadowaki, H. Sakai, K. Kobayashi, H. Horinouchi, T. Maruyama, M. Otagiri. Fluid resuscitation with hemoglobin vesicles prevents Esherichia coli growth via complement activation in a hemorrhagic shock rat model. J. Pharmacol. Exp. Therap. 337, 201-208 (2011).

D. Takahashi, H. Azuma, H. Sakai, K. Sou, D. Wakita, H. Abe, M. Fujihara, H. Horinouchi, K. Kobayashi, T. Nishimura, H. Ikeda. Phagocytosis of liposome particles by rat splenic immature monocytes makes them transiently and highly immunosuppressive in ex vivo culture condition. J. Pharmacol. Exp. Therap. 337, 42-49 (2011).

K. Taguchi, Y. Iwao, H. Watanabe, D. Kadowaki, H. Sakai, K. Kobayashi, H. Horinouchi, T. Maruyama, M. Otagiri. Repeated injection of high dose of hemoglobin encapsulated liposomes (hemoglobin-vesicles) induces accelerated blood clearance in a hemorrhagic shock rat model. Drug Metab. Dispos. 39, 484-489 (2011).

H. Sakai, N. Okuda, S. Takeoka, E. Tsuchida. Increased viscosity of hemoglobin-based oxygen carriers retards NO-binding when perfused through narrow gas-permeable tubes. Microvasc. Res. 81, 169-176 (2011).

K. Taguchi, M. Miyasato, H. Watanabe, H. Sakai, H. Horinouchi, K. Kobayashi, E. Tsuchida, T. Maruyama, M. Otagiri. Alteration in the pharmacokinetics of hemoglobin-vesicles in a rat model of chronic liver cirrhosis is associated with Kupffer cell phagocyte activity. J. Pharmaceut. Sci. 100, 775-783 (2011).

P.K. Ong, S. Jain, B. Namgung, Y.I. Woo, H. Sakai, D. Lim, K.J. Chun, S. Kim. An automated method for cell-free layer width determination in small arterioles. Physiol. Meas. 2011, 32, N1-N12 (2011).

H. Sakai, S. Takeoka, K. Kobayashi. Gas bioengineering using hemoglobin-vesicles for versatile clinical application. Current Pharmaceutical Design 17, 2352-2359 (2011).

H. Sakai. What is the major mechanism of slower NO uptake by red blood cells? (Letter to Editorial). J. Biol. Chem. 286, le22, (2011).

H.W. Kim, A. Mozzarelli, H. Sakai, J. Jahr. Academia-industry collaboration in blood substitute development. -issues, case histories and a proposal. In: Chemistry and Biochemistry of Oxygen Therapeutics: from Transfusion to Artificial Blood. (Ed. by S. Bettati and A. Mozzarelli), Chapter 29. pp. 413-428. John Wiley & Sons (2011).

H. Sakai, H. Horinouchi, E. Tsuchida, K. Kobayashi. Hemoglobin-vesicles as a cellular type hemoglobin-based oxygen carrier. In: Chemistry and Biochemistry of Oxygen Therapeutics: from Transfusion to Artificial Blood. (Ed. by S. Bettati and A. Mozzarelli), Chapter 27, pp.381-390. John Wiley & Sons (2011).

H. Sakai. Artificial oxygen carriers (hemoglobin-vesicles) as a transfusion alternative and for oxygen therapeutics. IFMBE Proceedings 35 (5th Kuala Lumpur International Conference on Biomedical Engineering 2011 (BIOMED 2011) 20-23 June 11, Kuala Lumpur, Malaysia), 845-848 (2011).

K. Yamamoto, H. Miyake, M. Kusunoki, S. Osaki, Steric hindrance by 2 amino acid residues determines the substrate specificity of isomaltase from Saccharomyces cerevisiae, J. Biosci. Bioeng., 112, 545-550 (2011).


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