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B. Takase, Y. Higashimura, N. Masaki, M. Kinoshita, T. Adachi, H. Sakai. Liposome-encapsulated hemoglobin rescues hemorrhagic shock heart through
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M. Otagiri, H. Watanabe, T. Maruyama. Carbon monoxide-loaded red blood
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I. Noguchi, H. Maeda, K. Kobayashi, T. Nagasaki, H. Kato, H. Yanagisawa,
N. Wada, G. Kanazawa, T. Kaji, H. Sakai, S. Fujimaki, Y. Ono, K. Taguchi, V. T. G. Chuang, J. Saruwatari, M. Otagiri,
H. Watanabe, T. Maruyama. Carbon monoxide-loaded cell therapy as an exercise
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K. Nakano, M. Kohno, H. Onozawa, R. Hashimoto, K. Oiwa, R. Masuda, M. Yamaguchi,
T. Hato, M. Watanabe, H. Horinouchi, H. Sakai, K. Kobayashi, M. Iwazaki. Using hemoglobin vesicles to treat operative hemorrhagic
shock after pneumonectomy in dog models: an experimental study. Biomed. Res. 45(2), 91-101 (2024).
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H. Sakai, T. Kure, N. Kobayashi, T. Ito, Y. Yamada, T. Yamada, R. Miyamoto, T. Imaizumi, J. Ando, T. Soga,
Y. Osanai, M. Ogawa, T. Shimizu, T. Ishida, H. Azuma, Absence of anaphylactic
reactions to injection of hemoglobin vesicles (artificial oxygen carriers)
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奥田千愛、酒井宏水. 人工赤血球を用いた一酸化炭素投与. Precision Medicine 7(10), 833-836 (2024).
松野直徒、石井大介、中條哲也、金子太樹、寺口博也、岡田陽子、鳥海飛鳥、小原弘道、暮地本宙也、稲永由紀子、李小康、酒井宏水．機械灌流保存が移植外科学にもたらしたもの - 臓器保存から臓器創出．Organ Biology 31(2) 1-4 (2024).
酒井宏水、鶴田啓亮. 備蓄・緊急投与が可能な人工赤血球の実用化に向けた研究開発. ICUとCCU 48(7) 375-379 (2024).
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Y. Syzuki, Y. Arakida, H. Sakai, Y. Enoki, K. Matsumoto, K. Taguchi, Elucidating the binding properties
of methemoglobin in red blood cell to cyanide, hydrosulfide, and azide
ions using artificial red blood cell. Toxycol. Appl. Pharmacol. 481(15), 116752 (2023).
DOI:10.1016/j.taap.2023.116752
T. Nagasai, H. Maeda, H. Yanagisawa, K. Nishida,K. Kobayashi, N. Wada,
I. Noguchi, R. Iwakiri, K. Taguchi, H. Sakai, J. Saruwatari, H. Watanabe, M. Otagiri, T. Maruyama, Carbon monoxide-loaded
red blood cell prevents the onset of cisplatin-induced acute kidney injury.
Antioxidants 12(9), 1705 (2023).
DOI:10.3390/antiox12091705
N. K. M. Zin, H. Bochimoto, D. Kondoh, Y. Ishihara, H. Iwata, T. Shonaka,
H. Obara, H. Sakai, H. Furukawa, N. Matsuno, Machine perfusion preservation with hemoglobin
based oxygen vesicles alleviate ultrastructural damages in porcine liver
donated after cardiac death. Microsc. Res. Tech. 86, 1725-1732 (2023).
DOI:10.1002/jemt.24405
T. Ochi, H. Nishiofuku, T. Kure, N. Saito, R. Taiji, N. Marugami, T. Tanaka, H. Sakai, Development of liposomal contrast agent with high iodine concentration
and minimal effect on renal function. Biochem. Biophys. Rep. 34, 101473 (2023).
DOI:10.1016/j.bbrep.2023.101473
B. Takase, Y. Higashimura, H. Asahina, M. Ishihara, H. Sakai, Cardiac autonomic activity in a hemorrhagic shock-induced rat heart model.
Curr. Med. Sci. 43, 232-245 (2023).
DOI:10.1007/s11596-023-2706-9
N. Rikihisa, K. Shimanouchi, Y. Saito, H. Sakai, N. Mitsukawa, Carbon monoxide combined with artificial blood cells acts
as an antioxidant for tissues that have been thermal-damaged by dye laser
irradiation. Burns 49(2), 388-400 (2023).
DOI:10.1016/j.burns.2022.03.009
髙瀬凡平、東村悠子、朝比奈はるか、林克、久田哲也、眞﨑暢之、木下学、永田雅良、酒井宏水. 動脈性出血性ショックモデルにおける人工赤血球(HbV)の心保護作用と抗不整脈作用に関する検討. Therapeutic Research 44(4), 249-255 (2023)．リンク
酒井宏水，小林直子，久禮智子，東寛．“人工赤血球”の外科・代謝栄養学への応用の可能性．外科と代謝・栄養 57(4), 100-103 (2023)．
DOI:10.11638/jssmn.57.4_100
松平崇，酒井宏水．ヘモグロビンとPEGが結合した巨大な環状モノマーの開環重合．化学と工業 76(4), 261 (2023)．リンク
H. Sakai, T. Kure, K. Taguchi, H. Azuma, Research of storable and ready-to-use artificial
red blood cells (hemoglobin vesicles) for emergency medicine and other
clinical applications. Front. Med. Technol. 4, 1048951 (2022).
DOI:10.3389/fmedt.2022.1048951
H. Sakai, S. Yasuda, C. Okuda, T. Yamada, K. Owaki, Y. Miwa, Examination of central nervous system by
functional observation battery after massive intravenous infusion of carbon
monoxide-bound and oxygen-bound hemoglobin vesicles in rats. CRPHAR 3, 100135 (2022).
DOI:10.1016/j.crphar.2022.100135
K. Yamamoto, H. Tanaka, G. Kurisu, R. Nakano, H. Yano, H. Sakai, Structural insights into the substrate specificity of IMP-6 and IMP-1
metallo-β-lactamases. J. Biochem. 173(1), 21-30 (2022).
DOI:10.1093/jb/mvac080
H. Onozawa, M. Kohno, R. Hashimoto, K. Oiwa, R. Masuda, M. Yamaguchi, T.
Hato, M. Watanabe, H. Horinouchi, H. Sakai, K. Kobayashi, M. Iwazaki, Early reperfusion with hemoglobin vesicles
into tracheal subepithelial capillaries in a mouse tracheal transplant
model. Biomed. Res. 43(4), 137-144 (2022).
DOI:10.2220/biomedres.43.137
H. Azuma, T. Amano, N. Kamiyama, N. Takehara, M. Jingu, H. Takagi, O. Sugita, N. Kobayashi, T. Kure, T. Shimizu, T. Ishida, M. Matsumoto, H. Sakai, First-in-human phase 1 trial of artificial red blood cells and hemoglobin
vesicles developed as a transfusion alternative. Blood Adv. 6(21), 5711-5715 (2022).
DOI:10.1182/bloodadvances.2022007977
S. Ghirmai, H. Wu, M. Axelsson, T. Matsuhira, H. Sakai, I. Undeland, Exploring how plasma- and muscle-related parameters affect
trout hemolysis as a route to prevent hemoglobin-mediated lipid oxidation
of fish muscle. Sci. Rep. 12, 13446 (2022).
DOI:10.1038/s41598-022-16363-4
Y. Suzuki, K. Taguchi, T. Kure, Y. Enoki, M. Otagiri, H. Sakai, K. Matsumoto, Liposomal methemoglobin as a potent antidote for hydrogen
sulfide poisoning. Toxicol. Appl. Pharmacol. 450(1), 116159 (2022).
DOI:10.1016/j.taap.2022.116159
T. Nagasaki, H. Maeda, K. Taguchi, H. Yanagisawa, K. Nishida, K. Kobayashi,
N. Wada, I. Noguchi, R. Murata, H. Sakai, H. Kitagishi, J. Saruwatari, H. Watanabe, M. Otagiri, T. Maruyama, A
bioinspired carbon monoxide delivery system prevents acute kidney injury
and the progression to chronic kidney disease. Redox Biology, 54, 102371 (2022).
DOI:10.1016/j.redox.2022.102371
Y. Yuki, K. Hasegawa, M. Kinoshita, H. Ishibashi, K. Kaneko, O. Ishida,
D. Saitoh, H. Sakai, K. Terui, Efficacy of resuscitative infusion with hemoglobin vesicles
in rabbits with massive obstetric hemorrhage. Obstet. Anesth. Dig. 42(1) 18-19 (2022).
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Y. Suzuki, K. Taguchi, S. Hanyu, T. Kure, Y. Enoki, M. Otagiri, H. Sakai, K. Matsumoto, Oxidized liposomal artificial red blood cells rescue azide-poisoned
mice from lethal toxidrome by recovering cytochrome c oxidase activity. J. Drug Deliv.Sci. Technol. 71, 103282 (2022).
DOI:10.1016/j.jddst.2022.103282
C. Okuda, H. Sakai, Effect of carbon monoxide administration using haemoglobin-vesicles on
the hippocampal tissue. Artif. Cells Nanomed, Biotech. 50(1), 1-9 (2022).
DOI:10.1080/21691401.2022.2027428
B. Takase, Y. Higashimura, H. Asahina, N. Masaki, M. Kinoshita, H. Sakai, Intraosseous infusion of liposome-encapsulated hemoglobin (HbV) acutely
prevents hemorrhagic anemia-induced lethal arrhythmias and its efficacy
persists with preventing proarrhythmic side effects in subacute phase of
severe hemodilution model. Artif. Organs 46(6), 1107-1121 (2022).
DOI:10.1111/aor.14170
K. Taguchi, Y. Suzuki, M. Tsutsuura, K. Hiraoka, Y. Watabe, Y. Enoki, M.
Otagiri, H. Sakai, K. Matsumoto, Liposomal Artificial Red Blood Cell-Based Carbon Monoxide
Donor Is a Potent Renoprotectant against Cisplatin-Induced Acute Kidney
Injury. Pharmaceutics, 14(1), 57 (2022).
DOI:10.3390/pharmaceutics14010057
H. Usuda, M. Saito, H. Ikeda, S. Sato, Y. Kumagai, Y. Saito, S. Kawamura,
T. Hanita, H. Sakai, S. Kure, N. Yaegashi, J. P. Newnham, M. W. Kemp, S. Watanabe, Assessment
of synthetic red cell therapy for extremely preterm ovine fetuses maintained
on an artificial placenta life-support platform. Artif. Organs 46(4), 653-665 (2022).
DOI:10.1111/aor.14155
Y. Watabe, K. Taguchi, H. Sakai, Y. Enoki, T. Maruyama, M. Otagiri, M Kohno, K. Matsumoto, Bioinspired
carbon monoxide delivery using artificial blood attenuates the progression
of obliterative bronchiolitis via suppression of macrophage activation
by IL-17A. Eur. J. Pharm. Biopharm. 170, 43-51 (2022).
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T. Matsuhira, H. Sakai, Artificial oxygen carriers, from nanometer- to micrometer-sized particles,
made of hemoglobin composites substituting for red blood cells. Particuology 64, 43-55 (2022).
DOI:10.1016/j.partic.2021.08.010
H. Sakai, N. Kobayashi, T. Kure, H. Azuma. Potential Clinical Application of Hemoglobin Vesicles as an
Artificial Oxygen Carrier and Carbon Monoxide Carrier. In: “Blood Substitutes
and Oxygen Biotherapeutics” (Eds. by H. Liu, A.D. Kaye, J.S. Jahr), Springer
Nature Switzerland AG. Switzerland, pp. 235-242 (2022).
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酒井宏水, 小林直子, 久禮智子. 備蓄・緊急投与が可能な人工赤血球製剤の開発状況. 日本産婦人科・新生児血液学会誌 31(2), 127-133 (2022).
川井正雄, 松平崇. 基礎生命化学 ー分子から見た私たちー, 2022/04/01, かきく書房.
ISBN-13:978-4-9912052-0-0
川井正雄，山本惠三．井本稔博士 卒寿の問い．化学と工業 75(3), 220-221 (2022)．リンク
酒井宏水．備蓄・緊急投与が可能な人工赤血球製剤の実用化を目指す研究. 膜(MEMBRANE) 47(5), 246-251 (2022)．
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H. Ishibashi, K. Hagisawa, M. Kinoshita, Y. Yuki, M. Miyamoto, T. Kure, H. Sakai, D. Saitoh, K. Terui, M. Takano, Resuscitative efficacy of hemoglobin
vesicles for severe postpartum hemorrhage in pregnant rabbits. Sci. Rep. 11, 22367 (2021).
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Y. Suzuki, K. Taguchi, T. Kure, Y. Enoki, M. Otagiri, H. Sakai, K. Matsumoto, Long-term pharmaceutical stability of liposome-encapsulated
methemoglobin as an antidote for cyanide poisoning. Int. J. Pharm. 610(15), 121260 (2021).
DOI:10.1016/j.ijpharm.2021.121260
Y. Suzuki, K. Taguchi, T. Kure, H. Sakai, Y. Enoki, M. Otagiri, K. Matsumoto, Liposome-encapsulated methemoglobin
as an antidote against cyanide poisoning. J. Control. Release 337, 59-70 (2021).
DOI:10.1016/j.jconrel.2021.07.015
T. Kure, H. Sakai, Preparation of Artificial Red Blood Cells (Hemoglobin Vesicles) Using
the Rotation-Revolution Mixer for High Encapsulation Efficiency. ACS Biomater. Sci. Eng. 7(6), 2835-2844 (2021).
DOI:10.1021/acsbiomaterials.1c00424
H. Sakai, N. Kobayashi, T.Kure, C. Okuda, Translational research of hemoglobin vesicles as a transfusion alternative.
Curr. Med. Chem. 29(3), 591-606 (2021).
DOI:10.2174/0929867328666210412130035
T. Matsuhira, H. Sakai, Entropy-driven supramolecular ring-opening polymerization
of a cyclic hemoglobin monomer for constructing a hemoglobin-PEG alternating
polymer with structural regularity. Biomacromolecules 22(5), 1944-1954 (2021).
DOI:10.1021/acs.biomac.1c00061
K. Hagisawa, M. Kinoshita, H. Sakai, S. Takeoka, Artificial blood transfusion. Physiology News 121, 22-25. DOI:10.36866/pn.121.22
https://static.physoc.org/app/uploads/2021/03/29093550/PN121_ONLINE_HR.pdf
K. Shimanouchi, N. Rikihisa, Y. Saito, N. Tsumura, H. Sakai, N. Mitsukawa, Artificial red blood cells increase large vessel wall damage
and decrease surrounding dermal tissue damage in a rabbit auricle model
after subsequent flashlamp‐pumped pulsed‐dye laser treatment. J. Dermatol. 48(5), 600-612 (2021).
DOI:10.1111/1346-8138.15805
(Chapter 3.9) Analysis of dimeric αβ subunit exchange between bis-PEGylated and native hemoglobins. pp 417-431.木下学，萩沢康介，石田治，斎藤大蔵，酒井宏水，武岡真司．人工血液～外傷治療における明日にかける橋～. 日本外傷学会誌 35(4), 275-282 (2021). リンク
T. Matsuhira, K. Yamamoto, H. Sakai
(Chapter 3.10) Ring-opening polymerization of hemoglobin based on supramolecular chemistry. pp 433-445.
T. Matsuhira, K. Yamamoto, H. Sakai
(Chapter 6.7) Translational research of hemoglobin vesicles (artificial red cells) for a transfusion alternative and O2/CO therapeutics. pp 811-822.
H. Sakai, H. Azuma, H. Horinouchi, K. Kobayashi
(Chapter 6.8) Hemoglobin vesicles as a transfusion alternative for perioperative hemorrhage in thoracic surgery. pp 823-835.
M. Kohno, R. Hashimoto, H. Onozawa, K. Oiwa, H. Horinouchi, H. Sakai, K. Kobayashi, M. Iwazaki
(Chapter 6.9) Prevention of methemoglobin formation in artificial red cells (hemoglobin vesicles). pp 837-847.
H. Sakai, M. Yamada
(Chapter 6.10) Carbon monoxide-bound hemoglobin vesicles: Current facts and potential medical applications. pp 849-866.
K. Taguchi, K. Matsumoto, H. Sakai, T. Maruyama, M. Otagiri
(Chapter 6.11) Influence of hemoglobin vesicles on immune function. pp 867-877.
H. Azuma, M. Fujihara, H. Sakai
(Chapter 6.12) Membrane fluidity of artificial red cells encapsulating a concentrated hemoglobin solution possessing a high colloid osmotic pressure. pp 879-888.
T. Kure, H. Sakai
Y. Yoshida, T. Nagamori, E. Ishibazawa, H. Kobayashi, T. Kure, H. Sakai, D. Takahashi, M. Fujihara, H. Azuma, Contribution of long chain fatty acid to induction of myeloid-derived suppressor cell (MDSC)-like cells - Induction of MDSC by lipid vesicles (liposome) - Immunopharmacol. Immunotoxicol. 42(6), 614-624 (2020). DOI:10.1080/08923973.2020.1837866
M. Tokuno, K. Taguchi, H. Sakai, S. Ohtsuki, K. Yamasaki, M. Otagiri, Assessing cytochrome P450-based drug-drug interactions with Hemoglobin-vesicles, an artificial red blood cell preparation, in healthy rats. Drug Metab. Pharmacokinet. 35(5), 425-431 (2020). DOI:10.1016/j.dmpk.2020.06.005
M. Tokuno, K. Taguchi, H. Sakai, S. Ohtsuki, K. Yamasaki, M. Otagiri, Evaluation of cytochrome P450-based drug metabolism in hemorrhagic shock rats that were transfused with native and an artificial red blood cell preparation, Hemoglobin-vesicles. Drug Metab. Pharmacokinet. 35(5), 417-424 (2020). DOI:10.1016/j.dmpk.2020.06.004
K. Hagisawa, M. Kinoshita, D. Saitoh, Y. Morimoto, H. Sakai, Intraosseous transfusion of hemoglobin vesicles in the treatment of hemorrhagic shock with collapsed vessels in a rabbit model. Transfusion 60(7), 1400-1409 (2020). DOI:10.1111/trf.15915
R. Hashimoto, M. Kohno, K. Oiwa, H. Onozawa, M. Watanabe, H. Horinouchi, H. Sakai, K. Kobayashi, M. Iwazaki, Immediate effects of systemic administration of normal and high O2-affinity haemoglobin vesicles as a transfusion alternative in a rat pneumonectomy model. BMJ Open Resp. Res. 7(1), e000476 (2020). DOI:10.1136/bmjresp-2019-000476
酒井宏水. 人工赤血球(ヘモグロビンベシクル)製剤による出血性ショック治療の可能性. 外科と代謝・栄養 54(4), 170-174 (2020).
DOI:10.11638/jssmn.54.4_170
田口和明, 酒井宏水. 一酸化炭素結合人工赤血球による出血性ショックからの蘇生. 循環制御 41(2), 95-96 (2020). リンク
石橋弘樹, 木下学, 萩沢康介, 酒井宏水, 宮本守員, 高野政志. 産科危機的出血をきたした妊娠ウサギに対する人工赤血球の効果. 循環制御 41(2), 89-91 (2020). リンク
山田孫平, 松平崇, 山本惠三, 酒井宏水. NADHとヘモグロビンの共存による抗酸化的な擬似酵素活性と人工赤血球への応用. 人工血液 28(1), 49-56 (2020). リンク
酒井宏水. 備蓄・緊急投与が可能な人工赤血球製剤の開発. Shock 34(2), 21-28 (2020). リンク
T. Shonaka, N. Matsuno, H. Obara, R. Yoshikawa, Y. Nishikawa, Y. Ishihara, H. Bochimoto, M. Gochi, M. Otani, H. Kanazawa, H. Azuma, H. Sakai, Hiroyuki Furukawa, Impact of human-derived hemoglobin based oxygen vesicles as a machine perfusion solution for liver donation after cardiac death in a pig model. PLOS ONE 14(12), e0226183 (2019). DOI:10.1371/journal.pone.0226183
H. Sakai, C. Leong, Prolonged functional life span of artificial red cells in blood circulation by repeated methylene blue injections. Artif. Cells Nanomed. Biotechnol. 47(1), 3123-3128 (2019). DOI:10.1080/21691401.2019.1645157
K. Hagisawa, M. Kinoshita, M. Takikawa, S. Takeoka, D. Saitoh, S. Seki, H. Sakai, Combination therapy using fibrinogen γ‐chain peptide‐coated, ADP‐encapsulated liposomes and hemoglobin vesicles for trauma‐induced massive hemorrhage in thrombocytopenic rabbits. Transfusion 59(10), 3186-3196 (2019). DOI:10.1111/trf.15427
M. Yamada, T. Matsuhira, K. Yamamoto, H. Sakai, Antioxidative pseudo-enzymatic mechanism of NAD(P)H coexisting with oxyhemoglobin for suppressed methemoglobin formation. Biochemistry 58(10), 1400-1410 (2019). DOI:10.1021/acs.biochem.8b01314
T. Matsuhira, K. Yamamoto, H. Sakai, Ring-opening polymerization of hemoglobin. Biomacromolecules 20(4), 1592-1602 (2019). DOI:10.1021/acs.biomac.8b01789
B. Takase, Y. Higashimura, K. Hashimoto, H. Asahina, M. Ishihara, H. Sakai, Myocardial Electrical Remodeling and the Arrhythmogenic Substrate in Hemorrhagic Shock-Induced Heart: Anti-Arrhythmogenic Effect of Liposome-Encapsulated Hemoglobin (HbV) on the Myocardium. Shock 52(3), 378-386 (2019). DOI: 10.1097/SHK.0000000000001262
高瀬凡平, 東村悠子, 朝比奈はるか, 酒井宏水. 出血性ショック心臓の催不整脈性作用に及ぼす人工赤血球の慢性効果. Therapeutics Research 40(9), 709-716 (2019). リンク
高瀬凡平, 橋本賢一, 東村悠子, 朝比奈はるか, 酒井宏水. 光工学的手法によるリポソーム封入酸素運搬体の致死性出血性ショック(85%出血後蘇生)実験モデルにおける抗不整脈効果に関する検討. Therapeutics Research 40(4), 227-284 (2019). リンク
東寛，酒井宏水. 人工赤血球（ヘモグロビンベシクル）の臨床への橋渡し研究. 臨床血液 60(9), 1084-1091 (2019). DOI:10.11406/rinketsu.60.1084
T. Matsuhira, T. Kure, K. Yamamoto, H. Sakai, Analysis of dimeric αβ subunit exchange between PEGylated and native hemoglobins (α2β2 tetramer) in an equilibrated state by intramolecular ββ-crosslinking. Biomacromolecules 19(8), 3412-3420 (2018). DOI: 10.1021/acs.biomac.8b00728
H. Sengyoku, T. Tsuchiya, T. Obata, R. Doi, Y. Hashimoto, M. Ishii, H. Sakai, N. Matsuo, D. Taniguchi, T. Suematsu, M. Lawn, K. Matsumoto, T. Miyazaki, T Nagayasu, Sodium hydroxide based non-detergent decellularizing solution for rat lung. Organogenesis 14(2), 94-106 (2018). DOI: 10.1080/15476278.2018.1462432
K. Taguchi, S. Nagao, H. Maeda, H. Yanagisawa, H. Sakai, K. Yamasaki, T. Wakayama, H. Watanabe, M. Otagiri, T. Maruyama, Biomimetic carbon monoxide delivery based on hemoglobin vesicles ameliorates acute pancreatitis in mice via the regulation of macrophage and neutrophil activity. Drug Delivery 25(1), 1266-1274 (2018). DOI: 10.1080/10717544.2018.1477860
N. Rikihisa, M. Tomonaga, S. Watanabe, Y. Saito, H. Sakai, Intravenous injection of artificial red cells and subsequent dye laser irradiation causes deep vessel impairment in an animal model of port-wine stain. Lasers Med. Sci. 33(6), 1287-1293 (2018). DOI: 10.1007/s10103-018-2480-2
T. Shonaka, N. Matsuno, H. Obara, R. Yoshikawa, Y. Nishikawa, M. Gouchi, M. Otani, H. Takahashi, H. Azuma, H. Sakai, H. Furukawa, The application of perfusate with human-derived oxygen carrier solution under subnormothermic machine perfusion for donation after cardiac death (DCD) liver grafts in pigs. Transp. Proc. 50(9), 2821-2825 (2018). DOI: 10.1016/j.transproceed.2018.02.184
K. Hagisawa, M. Kinoshita, B. Takase, K. Hashimoto, D. Saitoh, S. Seki, Y. Nishida, H. Sakai, Efficacy of Resuscitative Transfusion with Hemoglobin Vesicles in the Treatment of Massive Hemorrhage in Rabbits with Thrombocytopenic Coagulopathy and its Effect on Hemostasis by Platelet Transfusion. Shock 50(3), 324-330 (2018). DOI: 10.1097/SHK.0000000000001042
庄中達也，松野直徒，小原弘道，吉川遼，西川祐司，合地美香子，大谷将秀，高橋裕之，東寛，酒井宏水，古川博之. 心停止ドナーへの肝移植に対する人工赤血球(Hemoglobin vesicles, HbV)を用いた灌流保存方法の有効性. 北海道外科雑誌 63(2), 46-48 (2018).
酒井宏水，奥田千愛，久禮智子. 人工赤血球の開発と臨床応用. LiSA 25(11), 1139-1146 (2018).
酒井宏水. 輸血代替の他にもさまざまな利用が期待される「人工赤血球」とは. Medical Technology 46 (9), 832-834 (2018).
H. Sakai, Artificial red cells (hemoglobin vesicles) as a transfusion alternative and their new clinical applications. Japanese Journal of Transfusion and Cell Therapyt 64(4), 589-596 (2018). (in Japanese) 酒井宏水. 人工赤血球（ヘモグロビン ベシクル）製剤の新しい利用法. 日本輸血細胞治療学会雑誌 64(4), 589-596 (2018).
T. Kure, H. Sakai, The measurement of the lipid membrane fluidity of liposomes encapsulating a concentrated protein solution using a fluorescence polarization technique. Jasco report 60(2), 5-12 (2018). (in Japanese) 久禮智子，酒井宏水. 蛍光偏光解消法による高濃度蛋白質溶液を内包したリポソーム脂質膜の流動性評価. Jasco Report 60(2), 5-12 (2018).
酒井宏水. 廃棄する血液から人工赤血球をつくる。長期保存可能、献血不足の課題解決に. コンバーテック 541, 20-23 (2018).
酒井宏水，太田英伸，小林紘一，八重樫伸生. 人工赤血球（ヘモグロビンベシクル）. 日本産婦人科医会、研修ノートNo. 100「産婦人科医療の近未来」 67-69 (2018年 1月).
S. Ghirmai, L. Bulow, H. Sakai, In vivo evaluation of electron mediators for the reduction of methemoglobin encapsulated in liposomes using electron energies produced by red blood cell glycolysis. Artif. Cells Nanomed. Biotechnol. 46, 1364-1372 (2017). DOI: 10.1080/21691401.2017.1397003
H. Ohta, M. Kaga, H. Li, H. Sakai, K. Okamura, N. Yaegashi, Potential New Non-Invasive Therapy Using Artificial Oxygen Carriers for Pre-Eclampsia. J. Funct. Biomater. 8, 32 (2017). DOI: 10.3390/jfb8030032
H. Azuma, M. Fujihara, H. Sakai, Biocompatibility of HbV: Liposome-Encapsulated Hemoglobin Molecules-Liposome Effects on Immune Function. J. Funct. Biomater. 8, 24 (2017). DOI: 10.3390/jfb8030024
M. Kohno, T. Ikeda, R. Hashimoto, Y. Izumi, M. Watanabe, H. Horinouchi, H. Sakai, K. Kobayashi, M. Iwazaki, Acute 40% exchange-transfusion with hemoglobin-vesicles in a mouse pneumonectomy model. PLOS ONE 12(6), e0179724 (2017). DOI: 10.1371/journal.pone.0178724
M. Yamada, H. Sakai. Hidden Antioxidative Functions of NADH Coexisting with Hemoglobin. ACS Chem. Biol. 12(7), 1820-1829 (2017). DOI: 10.1021/acschembio.7b00174
K. Taguchi, K. Yamasaki, H. Sakai, T. Maruyama, M. Otagiri, The use of Hemoglobin-vesicles for delivering medicinal gas for the treatment of intractable disorders. J. Pharmaceut. Sci. 106(9), 2392-2400 (2017). DOI: 10.1016/j.xphs.2017.04.006
H. Azuma, Y, Yoshida, H. Takahashi, E. Ishibazawa, H. Kobayashi, H. Sakai, D. Takahashi, M. Fujihara. Liposomal microparticle injection can induce myeloid-derived suppressor cells (MDSC)-like cells in vivo. Immunopharmacol. Immunotoxicol. 39, 140-147 (2017). DOI: 10.1080/08923973.2017.1306867
N. Rikihisa, S. Watanabe, Y. Saito, H. Sakai. Artificial Red Blood Cells as Potential Photosensitizers in Dye Laser Treatment Against Port-Wine Stains. J. Funct. Biomater. 8, 14, (2017). DOI: 10.3390/jfb8020014
H. Sakai. Overview of potential clinical applications of hemoglobin vesicles (HbV) as artificial red cells, evidenced by preclinical studies of the academic research consortium. J. Funct. Biomater. 8, 10 (2017). DOI: 10.3390/jfb8010010
Y. Naito, H. Sakai, S. Inoue, M. Kawaguchi. Hemoglobin vesicles prolong the time to circulatory collapse in rats during apnea. BMC Anesthesiol. 17, 44 (2017). DOI: 10.1186/s12871-017-0338-y
N. Rikihisa, S. Watanabe, K. Satoh, Y. Saito, H. Sakai. Photosensitizer Effects of Artificial Red Cells on Dye Laser Irradiation in an Animal Model Assuming Port-Wine Stain Treatment. Plast. Reconstr. Surg. 139, 707e-716e (2017). DOI: 10.1097/PRS.0000000000003082
T. Kure, H. Sakai. Transmembrane difference in colloid osmotic pressure affects the lipid membrane fluidity of liposomes encapsulating a concentrated hemoglobin solution. Langmuir 33, 1533-1540 (2017). DOI: 10.1021/acs.langmuir.6b04643
M. Tokuno, K. Taguchi, K. Yamasaki, H. Sakai, M. Otagiri. Long-term stored hemoglobin-vesicles, a cellular type of hemoglobin-based oxygen carrier, has resuscitative effects comparable to that for fresh red blood cells in a rat model with massive hemorrhage without post-transfusion lung injury. PLoS ONE 11(10), e0165557 (2016).
S. Nagao, K. Taguchi, H. Sakai, K. Yamasaki, H. Watanabe, M. Otagiri, T. Maruyama. Carbon monoxide-bound hemoglobin vesicles ameliorate multiorgan injuries induced by severe acute pancreatitis in mice by their anti-inflammatory and antioxidant properties. Int. J. Nanomedicine 11, 5611-5620 (2016).
S. Nagao, K. Taguchi, Y. Miyazaki, T. Wakayama T, VT. Chuang, K. Yamasaki, H. Watanabe, H. Sakai, M. Otagiri, T. Maruyama. Evaluation of a new type of nano-sized carbon monoxide donor on treating mice with experimentally induced colitis. J. Control. Release 234, 49-58 (2016).
S. Osaki, K. Yamamoto, T. Matsuhira, H. Sakai. The effects of seasonal changes on the molecular weight of Nephila clavata spider silk. Polymer Journal 48, 659-653 (2016). DOI: 10.1038/pj.2015.138
酒井宏水(翻訳分担). ハーバー・生化学（原書30版）2016/11/30 丸善 53章(799-811) 清水孝雄(監訳).
太田英伸，加賀麻衣子，李コウ，酒井宏水，岡村州博，八重樫伸生. 人工酸素運搬体は妊娠高血圧症候群ラットモデルの胎盤低酸素・胎仔発育不全を治療する. 人工血液 24, 63-72 (2016).
B. Namgung, H. Sakai, S. Kim. Influence of erythrocyte aggregation at pathological levels on cell-free
marginal layer in a narrow circular tube. Clin. Hemorheol. Microcirculation 61, 445-457 (2015).
H. Li, H. Ohta, Y. Tahara, S. Nakamura, K. Taguchi, M. Nakagawa, Y. Oishi,
Y. Goto, K. Wada, M. Kaga, M. Inagaki, M. Otagiri, H. Yokota, S. Shibata,
H. Sakai, K. Okamura, N. Yaegashi. Artificial oxygen carriers rescue placental hypoxia and improve fetal development
in the rat pre-eclampsia model. Sci. Rep. 5, 15271 (2015).
K. Taguchi, S. Nagao, K. Yamasaki, H. Sakai, H. Seo, T. Maruyama, M. Otagiri. Biological Responsiveness and Metabolic Performance of Liposome-Encapsulated Hemoglobin (Hemoglobin-Vesicles) in Apolipoprotein E-Deficient Mice after Massive Intravenous Injection. Biol. Pharm. Bull. 38, 1606-1616 (2015).
K. Kettisen, L. Bulow, H. Sakai. Potential electron mediators to extract electron energies of RBC glycolysis for prolonged in vivo functional lifetime of hemoglobin-vesicles. Bioconjugate Chem. 26, 746-754 (2015).
J. Araki, H. Sakai, D. Takeuchi, Y. Kagaya, M. Naito, M. Mihara, M. Narushima, T. Iida, I. Koshima. Normothermic preservation of the rat hind limb with artificial oxygen-carrying hemoglobin vesicles. Transplantation 99, 687-692 (2015).
T. Matsuhira, S. Osaki. Molecular weight of Nephila clavata spider silk. Polymer Journal, 47, 456-459 (2015). DOI: 10.1038/pj.2015.10
酒井宏水, 久禮智子. 人工赤血球(ヘモグロビンベシクル)の実現に向けて. 医学のあゆみ 253, 1180-1181 (2015).
S. Nagao, K. Taguchi, H. Sakai, R. Tanaka, H. Horinouchi, H. Watanabe, K. Kobayashi, M. Otagiri, T. Maruyama.
Carbon monoxide-bound hemoglobin-vesicles as a potential therapeutic agent
for the treatment of bleomycin-induced pulmonary fibrosis. Biomaterials 35, 6553-6562 (2014)
M. Fujihara, D. Takahashi, H. Abe, H. Sakai, H. Horinouchi, K. Kobayashi, H. Ikeda, H. Azuma. Primary and secondary immune responses to keyhole limpet hemocyanin in
rats after the infusion of hemoglobin vesicle, an artificial oxygen carrier. Artif. Organs 38, 234-238 (2014)
酒井宏水, 人工赤血球(ヘモグロビン小胞体)微粒子分散液の特徴. 粉体工学 6, 909-914 (2014).
酒井宏水, 人工赤血球による生体組織への酸素輸送. 「全人力・科学力・透析力に基づく透析医学」第6章: 腎性貧血. pp.369-373、平方秀樹 監修、医薬ジャーナル社. 大阪 (2014).
酒井宏水, 人工赤血球の開発状況と将来展望. Anesthesia Network 18, 37-41 (2014).
H. Sakai, K. Ng, B. Li, N. Sugimura. Swine hemoglobin as a potential source of artificial oxygen carriers, hemoglobin-vesicles. Artif. Cells Nanomed. Biotechnol. 41, 37-41 (2013).
H.W. Kim, J.S. Jahr, A. Mozzarelli, H. Sakai. International consortium for development of hemoglobin-based oxygen carriers, oxygen therapeutics and multifunctional resuscitation fluids-a white paper. In: Hemoglobin-based oxygen carriers-principles, approaches and current status (H.W. Kim & A.G. Greenburg eds.) Springer-Verlag (Berlin/Heidelberg, Germany). Chapter 39, pp. 737-746 (2013).
T. Ikeda, H. Horinouchi, H. Sakai. Cellular-type hemoglobin-based oxygen carriers as a resuscitative fluid for hemorrhagic shock: acute and long-term safety evaluation using beagle dogs. In: Hemoglobin-based oxygen carriers-principles, approaches and current status (H.W. Kim & A.G. Greenburg eds.) Springer-Verlag (Berlin/Heidelberg, Germany). Chapter 28, pp. 501-526 (2013).
H. Azuma, M. Fujihara, H. Sakai. Biocompatibility of hemoglobin vesicles, a cellular-type artificial oxygen
carrier, on blood cells and plasma proteins in vitro and in vivo. In: Hemoglobin-based oxygen carriers-principles, approaches and current status
(H.W. Kim & A.G. Greenburg eds.) Springer-Verlag (Berlin/Heidelberg,
Germany). Chapter 22, pp. 385-398 (2013).
H. Sakai. Cellular-type hemoglobin-based oxygen carriers to mimic the red blood
cells structure. In: Hemoglobin-based oxygen carriers-principles, approaches and current status
(H.W. Kim & A.G. Greenburg eds.) Springer-Verlag (Berlin/Heidelberg,
Germany). Chapter 12, pp. 235-248 (2013).
酒井宏水，堀之内宏久、東寛、小田切優樹、小林紘一. 輸血代替としての人工赤血球(ヘモグロビン小胞体)製剤の安全性試験. 人工血液 21, 36-48 (2013).
H. Sakai. Biocompatibility of a highly concentrated fluid of Hemoglobin-vesicles
as a transfusion alternative. In: Selective Topics in Nanomedicine (T.M.S. Chang ed.), pp. 133-147, World Scientific, Singapore (2013).
T. Matsuhira, K. Yamamoto, S. Osaki. Effects of UV irradiation on the molecular weight of spider silk, Polymer Journal, 45, 1167-1169 (2013). DOI: 10.1038/pj.2013.41
K. Taguchi, H. Watanabe, H. Sakai, H. Horinouchi, K. Kobayashi, T. Maruyama, M. Otagiri. Fourteen-days observation
and pharmacokinetic evaluation after massive intravenous infusion of hemoglobin-vesicles
(artificial oxygen carriers) in cynomolgus monkeys. J. Drug Metab. Toxicol. 3, 1000128 (2012).
M. Kaga, H. Ohta, Y. Lee, R. Kamii, H. Yamamoto, S. Akiyama, S. Watanabe,
T. Matsuda, Y. Kimura, S. Tsuchiya, H. Tei, L. Okamura, H. Sakai, N. Yaegashi. Physiolgical capacity of the reticuloendothelial system for
the degradation of hemoglobin-vesicles (artificial oxygen carriers) after
massive intravenous doses by daily repeated infusion for 7 days in Pregnant
rats and fetuses. Life Sci. 91, 420-428 (2012).
H. Sakai, Y. Suzuki, K. Sou, M. Kano. Cardiopulmonary hemodynamic responses to
the small injection of hemoglobin-vesicles (artificial oxygen carriers)
in miniature pigs. J. Biomed. Mater. Res. A, 100A, 2668-2677 (2012).
A.G. Tsai, M. Intaglietta, H. Sakai, E. Delpy, C.D. la Rochelle, M. Rousselot, F. Zal. Microcirculation and
NO-CO studies of a natural extracellular hemoglobin developed for an oxygen
therapeutic carrier. Current Drug Discovery Techonl. 9, 166-172 (2012).
Y. Seishi, H. Horinouchi, H. Sakai, K. Kobayashi. Effect of the cellular-type artificial oxygen carrier Hb-vesicle
as a resuscitative fluid for pre-hospital treatment: Experiments in a rat
uncontrolled hemorrhagic shock model. Shock 38, 153-158 (2012).
H. Sakai, K. Sou, H. Horinouchi, E. Tsuchida, K. Kobayashi. Removal of Hemoglobin-vesicles
from circulating blood by using centrifugation and ultrafiltration. Artif. Organs 36, 202-209 (2012).
M. Yamamoto, H. Horinouchi, Y. Seishi, N. Sato, M. Itoh, K. Kobayashi,
H. Sakai, Fluid resuscitation of hemorrhagic shock with Hemoglobin vesicles in
Beagle dogs: pilot study. Artif. Cells Blood Substitutes Biotechnol. 40, 179-195 (2012).
T. Sato, T. Fukasawa, T. Komatsu, H. Sakai, S. Ishiwata. Protein-protein interactions in solution and their interplay
with protein specific functions. J. Phys. Soc. Jpn 81 (suppl.), SA002-1 - SA-002-11 (2012)
H. Sakai. Cellular-type hemoglobin-based oxygen carrier (hemoglobin-vesicles) as a transfusion alternative and for oxygen therapeutics. Current Drug Discovery Techonl. 9, 188-193 (2012).
H. Sakai, N. Miyagawa, H. Horinouchi, S. Takeoka, M. Takaori, E. Tsuchida, K. Kobayashi.
Intravenous injection of Hb-vesicles (artificial oxygen carriers) after
hemodilution with a series of plasma expanders (water-soluble biopolymers)
in a rat repeated hemorrhage model. Polymers Adv. Technol. 22, 1216-1222 (2011).
A. Niwa, T. Heike, K. Umeda, K. Oshima, I. Kato, H. Sakai, H. Suemori, T. Nakahata, M.K. Saito. A novel serum-free monolayer culture
for orderly hematopoietic differentiation of human pluripotent cells via
mesodermal progenitors". PLoS ONE 6, e22261 (2011).
K. Taguchi, S. Ogaki, H. Watanabe, D. Kadowaki, H. Sakai, K. Kobayashi, H. Horinouchi, T. Maruyama, M. Otagiri. Fluid resuscitation
with hemoglobin vesicles prevents Esherichia coli growth via complement
activation in a hemorrhagic shock rat model. J. Pharmacol. Exp. Therap. 337, 201-208 (2011).
D. Takahashi, H. Azuma, H. Sakai, K. Sou, D. Wakita, H. Abe, M. Fujihara, H. Horinouchi, K. Kobayashi,
T. Nishimura, H. Ikeda. Phagocytosis of liposome particles by rat splenic
immature monocytes makes them transiently and highly immunosuppressive
in ex vivo culture condition. J. Pharmacol. Exp. Therap. 337, 42-49 (2011).
K. Taguchi, Y. Iwao, H. Watanabe, D. Kadowaki, H. Sakai, K. Kobayashi, H. Horinouchi, T. Maruyama, M. Otagiri. Repeated injection
of high dose of hemoglobin encapsulated liposomes (hemoglobin-vesicles)
induces accelerated blood clearance in a hemorrhagic shock rat model. Drug Metab. Dispos. 39, 484-489 (2011).
H. Sakai, N. Okuda, S. Takeoka, E. Tsuchida. Increased viscosity of hemoglobin-based oxygen carriers retards NO-binding
when perfused through narrow gas-permeable tubes. Microvasc. Res. 81, 169-176 (2011).
K. Taguchi, M. Miyasato, H. Watanabe, H. Sakai, H. Horinouchi, K. Kobayashi, E. Tsuchida, T. Maruyama, M. Otagiri. Alteration
in the pharmacokinetics of hemoglobin-vesicles in a rat model of chronic
liver cirrhosis is associated with Kupffer cell phagocyte activity. J. Pharmaceut. Sci. 100, 775-783 (2011).
P.K. Ong, S. Jain, B. Namgung, Y.I. Woo, H. Sakai, D. Lim, K.J. Chun, S. Kim. An automated method for cell-free layer width
determination in small arterioles. Physiol. Meas. 2011, 32, N1-N12 (2011).
H. Sakai, S. Takeoka, K. Kobayashi. Gas bioengineering using hemoglobin-vesicles
for versatile clinical application. Current Pharmaceutical Design 17, 2352-2359 (2011).
H. Sakai. What is the major mechanism of slower NO uptake by red blood cells? (Letter
to Editorial). J. Biol. Chem. 286, le22, (2011).
H.W. Kim, A. Mozzarelli, H. Sakai, J. Jahr. Academia-industry collaboration in blood substitute development.
-issues, case histories and a proposal. In: Chemistry and Biochemistry
of Oxygen Therapeutics: from Transfusion to Artificial Blood. (Ed. by S.
Bettati and A. Mozzarelli), Chapter 29. pp. 413-428. John Wiley & Sons
(2011).
H. Sakai, H. Horinouchi, E. Tsuchida, K. Kobayashi. Hemoglobin-vesicles as a cellular
type hemoglobin-based oxygen carrier. In: Chemistry and Biochemistry of
Oxygen Therapeutics: from Transfusion to Artificial Blood. (Ed. by S. Bettati
and A. Mozzarelli), Chapter 27, pp.381-390. John Wiley & Sons (2011).
H. Sakai. Artificial oxygen carriers (hemoglobin-vesicles) as a transfusion alternative
and for oxygen therapeutics. IFMBE Proceedings 35 (5th Kuala Lumpur International Conference on Biomedical Engineering 2011 (BIOMED
2011) 20-23 June 11, Kuala Lumpur, Malaysia), 845-848 (2011).
K. Yamamoto, H. Miyake, M. Kusunoki, S. Osaki, Steric hindrance by 2 amino acid residues determines the substrate specificity of isomaltase from Saccharomyces cerevisiae, J. Biosci. Bioeng., 112, 545-550 (2011).
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